Sherstennikova Aleksandra Konstantinovna, Associate professor, sub-department of normal physiology, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: firstname.lastname@example.org
Kashutin Sergey Leonidovich, Doctor of medical sciences, professor, head of sub-department of skin and venereal diseases, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: email@example.com
Kalmin Oleg Vital'evich, Doctor of medical sciences, professor, head of sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), E-mail: firstname.lastname@example.org
Neklyudova Viktorya Sergeevna, Assistant, sub-department of skin and venereal diseases, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: email@example.com
Background. Macrophages migrate to different tissues of the body, taking an active part in the early inflammatory response to infection and in triggering specific immune response. Information about the monocytes participation in the development of psoriatic process has a practical interest. The aim of this study was to examine the ratio of monocytes in the peripheral blood and blood of psoriatic papules with regard to their morphological features.
Materials and methods. Clinical and immunological examination of 89 patients with vulgar and exudative psoriasis (41 women and 48 men) aged 20 to 60 years was carried out. The concentration of monocytes among other leukocytes was determined on the smears of peripheral blood, and blood from the surface of psoriatic papules, fixed in a mixture of Nikiforov and stained by Romanovsky-Giemsa. Monocytes were investigated in accordance with their morphological properties (O. P. Grigorova). The content of L-selectin, LFA-1, LFA-3, ICAM-1, PECAM-1 was measured on a flow cytometer FC-500 from Beckman Coulter (USA) on neutrophils of whole blood.
Results. In the blood secreted from the surface of psoriatic papules compared with peripheral blood, the content of monocytes increased 3 times due to an increase in the level of polymorphonuclear forms against the background of a sharp decrease in the concentration of promonocytes. Confirmation of the predominant migration of polymorphonuclear monocytes was the presence of statistically significant correlations with the level of expression of adhesion molecules, especially those involved in the "transmigration" stage.
Conclusions. Mainly polymorphonuclear monocytes migrate into psoriatic papules, as evidenced by their accumulation in the lesion. Monocytes capable of proliferation do not migrate because the median content of their psoriatic papule is 0, and in venous blood their level is significantly higher than in the control group.
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